Three-time champion, Serena Williams, was stunned in the French Open third round by unseeded American, Sofia Kenin on Saturday.
The 10th seed lost lost 6-2 7-5 to her 20-year-old compatriot, who was booed by the crowd during the match.
The defeat means Serena’s quest to equal Margaret Court’s record of 24 Grand Slam singles titles continues.
“I’m so happy with this win – obviously you can tell with these emotions,” a crying Kenin told the crowd on Philippe Chatrier.
“Playing against Serena, you have to fight for every point.”
Serena’s defeat followed the shocking exit of world number one, Naomi Osaka, which now leaves only three top-10 players in the women’s draw.
Earlier in the day, defending champion, Simona Halep enjoyed a 55-minute victory over Ukraine’s Lesia Tsurenko, winning 6-2 6-1 to reach the last 16.
Kenin, who is ranked number 35 in the world, will now play Australian eighth seed, Ashleigh Barty in the fourth round, while Halep faces unseeded 18-year-old French Open debutant, Iga Swiatek.